Sticking it out with tight junctions
Sticking it out with tight junctions 【关键词】 sticking, it was enough to jump-start the field of tight junction biochemistry, some 20 years after the first morphological description of these junctions, which give epithelial cells their ability to seal off body compartments (farquhar and palade, 1963). the preparations yielded multiple protein bands―two major and six minor. from band to protein stevenson took the project with him to mark mooseker"s lab at yale university (new haven, ct) as a postdoc and collaborated with janet siliciano in the goodenough lab. using the tight junction fraction, they screened monoclonal antibodies by looking for localization to the junctions by immunofluorescence em. commuting back up to boston in his ford fiesta, stevenson and siliciano worked on the tedious process of antibody production. at first, some of their hybridomas would turn up positive, only to be lost upon cloning out to a 96-well plate. on a saturday before heading out to the beach, stevenson says he remembers checking two plates, and being prepared to throw them out, only to find all of the 48 wells turning up positive. he cancelled his vacation and set to cloning the colonies out for the rest of the day. his perseverance led to the antibody that identified the first tight junction protein, zo-1 (stevenson et al., 1986). the protein occurred in many other epithelial cell tight junctions including those of rat liver and mouse colon, kidney, and testis. the widespread distribution of the protein, cloned a few years later (anderson et al., 1989), argued that it could be a "ubiquitous component of all mammalian tight junctions." more than zo-1 but it was clear early on that, as a peripheral membrane protein, zo-1 was not the integral junction-forming protein. finding this protein was still up for grabs, and those in the united states went back to their tight junction preps to try to generate other antibodies. but contamination with the very antigenic zo-1 and gap junction proteins foiled those efforts. in the end, the prize went to shoichiro tsukita"s group, then at the national institute for physiological sciences in okazaki, japan. "tsukita," says goodenough, "had a wonderful insight" in switching species and using chicken as the source of antigen. tsukita recalls that by 1985 the problem of isolating the tight junction integral membrane protein had become so notorious that a joke was going around: "the boss should not mention this theme for postdoctoral fellows." he and his wife, sachiko, had established a procedure to isolate cadherin-based adherens junctions from rat livers (tsukita and tsukita, 1989) and were studying the proteins enriched in this fraction. immunizing mice with this fraction would ultimately show that the adherens junctions were contaminated with a large supply of zo-1 (itoh et al., 1993), which, tsukitareasoned, meant the preps were highly enriched for tight junctions. but in three years of raising monoclonal antibodies with the prep, he says, "we did not obtain one that appeared to recognize integral membrane tight junction proteins. what did this mean" one possible explanation was that tight junctions were formed by lipids, as some investigators had proposed. tsukita"s group, however, "believed in the ‘protein theory,’ based on stevenson"s pioneering work." another explanation was that the rat junctions were not sufficiently immunogenic in mice. new models;new proteins based on this latter idea, then graduate students mikio furuse and toshiaki hirase began isolating similar fractions from cows, pigs, hamsters, and hens. their attempts established the "furuse-hirase rule" of the lab: the smaller the livers, the more pure were the junction fractions. thus, newborn chick livers gave "very beautiful fractions." in the end, it would take 5,000 chick livers. "luckily, okazaki provides about 80% of japan"s chickens, so we got a very low price," says tsukita. zo-1 (top) and occludin (bottom) both localize to tight junctions. stevenson/tsukita using this tight junction fraction, the team generated three monoclonal antibodies that recognized a 65-kd integral membrane protein―a protein that localized to endothelial and epithelial tight junctions (furuse et al., 1993). the group cloned the protein, designated occludin, and depicted a model of the protein as having four transmembrane domains. using the chicken cdna of occludin to track down the mammalian occludins took another two years (ando-akatsuka et al., 1996). when tsukita"s group, now based at kyoto university school of medicine in japan, created mouse epithelial cells lacking occludin, however, the cells still formed tight junctions (saitou et al., 1998). this sent them back to the hunt for other integral membrane proteins from the tight junction fraction, using occludin as a probe. in 1998, furuse, tsukita, and colleagues identified the claudins, which also have four transmembrane domains but no sequence similarity to occludin (furuse et al., 1998). tsukita adds, "the identification of zo-1, occludin, and claudins opened a new way to understand the barrier and fence properties of tight junctions in molecular terms." anderson, j.m., et al. 1989. j. cell biol. 109:10471056. ando-akatsuka, y., et al. 1996. j. cell biol. 133:4347. farquhar, m.g., and g.e. palade. 1963. j. cell biol. 17:375412. furuse, m., et al. 1993. j. cell biol. 123:17771788. furuse, m., et al. 1998. j. cell biol. 141:15391550. itoh, m., et al. 1993. j. cell biol. 121:491502.saitou, m., et al. 1998. j. cell biol. 141:397408. stevenson, b.r., and d.a. goodenough. 1984. j. cell biol. 98:12091221. stevenson, b.r., et al. 1986. j. cell biol. 103:755766. tsukita, s., and s. tsukita. 1989. j. cell biol. 108:3141.