[非小细胞肺癌中Reprimo以及1433,Σ基因启动子异]primo re

非小细胞肺癌中Reprimo以及1433 Σ基因启动子异

非小细胞肺癌中Reprimo以及14  3  3 Σ基因启动子异 【摘要】 目的探讨reprimo以及14  3  3 σ基因启动子在非小细胞肺癌 （nsclc）中的异常甲基化状态及其与临床病理资料的联系。方法采用甲基化特异 性聚合酶链反应（methylation specific  pcr,msp）技术检测60例nsclc以及癌旁正 常组织中reprimo以及14  3  3 σ基因启动子异常甲基化状态。结果reprimo以及 14  3  3 σ基因启动子异常甲基化在nsclc组织中发生频率分别为36.67％(22/60) 和28.33%(17/60)，与癌旁正常组织具有显著性差异（p值分别为0.000和0.002）。

reprimo基因启动子异常甲基化频率与吸烟习惯以及年龄相关；
14  3  3 σ启动子 异常甲基化与年龄、吸烟习惯、性别、病理类型和临床分期以及淋巴结转移等无 相关。结论nsclc中存在reprimo和14  3  3 σ等基因启动子较高频率的异常甲基化。

【关键词】 肺癌;甲基化;reprimo基因;14  3  3 σ基因 detection of aberrant methylation of reprimo and 14  3  3 σ genes in non  small cell lung cancer jiang rui，yu shi  ying cancer center of tongji hospital, tongji medical college, huazhong science and technology university, wuhan 430030, china corresponding author: yu shi  ying, e  mail: syyu@tjh.tjmu.edu.cn abstract：objective to investigate the frequency of aberrant methylation of reprimo and 14  3  3 σ in non  small cell lung cancer (nsclc).methodsaberrant methylation of reprimo and 14  3  3 σ were examined by methylation specific  pcr (msp) in primary nsclc and normal lung tissues (n = 60).resultsaberrant methylation of reprimo and 14  3  3 σ were found to be present in 36.67％ and 28.33% of all nsclc patients respectively.three cases of aberrant methylation of reprimo(5%) and 4 cases of 14  3  3 σ(6.67%) were detected in normal lung tissues. the frequencies of aberrant methylation of reprimo and 14  3  3 σ between primary nsclc and normal lung tissue group were significantly different. aberrant methylation of reprimo was more frequently observed in older patients group (p=0.047) and smoking group (p=0.038). there was no significant correlation between reprimo methylation frequencies and sex, pathology type, clinical staging and lymphatic metastasis. similarly no significant association was found between 14  3  3 σ methylation frequencies and sex, age, pathology type, clinical staging, smoking habit and lymphatic metastasis.conclusionmethylation of reprimo and 14  3  3 σ may play an important role in the pathogenesis of nsclc. key words：lung cancer;
methylation;
reprimo;
14  3  3 σ tab 1primer sequence of reprimo and 14  3  3σgeneforward primerreverseprimerproduct14  3  3σmtggtagtttttatgaaaggcgtccctctaaccgcccaccacg105bp14  3  3σuatggtagtttttatgaaaggtgttccctctaaccacccaccaca107bpreprimomgcgagtgagcgtttagttcta cctaaaaccgaattcatcg112bpreprimouttgtgagtgagtgtttagtttgtaattacctaaaaccaaattcatc112bp 1资料与方法 1.1资料 1.1.1病理标本60例nsclc组织以及相应的癌旁无瘤肺组织标本来自华中科 技大学附属同济医院2005~2006年肺癌手术切除患者，组织经短时福尔马林固定 后取材，放入-20℃冰箱保存备用。Www.133229.cOm肿瘤组织采集时避开肿瘤 的液化坏死部分，对应的癌旁无瘤组织标本取材自距离肿瘤组织5cm以上的正常 肺组织。60例患者术前均未进行放、化疗。其中男41例，女19例，年龄34~73岁， 平均年龄53.6岁。肿瘤组织均经过病理学证实。按who标准分类，鳞癌32例，腺 癌28例。ⅰ期15例，ⅱ期26例，ⅲ期19例。淋巴结转移22例，未发生淋巴结转移 38例。

1.1.2试剂盒和试剂dna提取试剂盒以及50bp dna ladder marker购自tiangan 公司，dna 修饰试剂盒购自chemicon 公司，dna 聚合酶购自大连宝生物takara公 司，sssⅰ甲基转移酶购自美国neb公司。